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KMID : 0357419950250020235
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Korean journal of Virology
1995 Volume.25 No. 2 p.235 ~ p.242
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Overexpression of Sulfated Glycoprotein-2ISGP-2) mRNA in the Brain of IM Mouse Infected with 87V Scrapie Agent
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양형모
민본홍/최은경/김용선
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Abstract
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Scrapie-induced amyloid plaque formation in mouse is very useful animal model in research of human Alzheimer's disease. It has been suggested that sulfated glycoprotein-2(SGP2) is involved in neuronal cell death. In this study, we have
investigated
the
role of SGP-2 in formation of amyloid plaques in brains of IM mouse infected with 87V scrapie agent. Both control and scrapie-injected mice were sacrificed after 300 days incubation period and total cellular RNAs were extracted from cerebral
cortex,
brain stem, cerebellum and entire brain. Northern blot analysis showed that SGP-2 mRNA label was significantly increased in brains of scrapie-infected mice than in control mice. In dissected tissue samples, the level of SGP-2 mRNA was elevated in
cerebellum and brain stem but not in cerebral cortex. Since amyloid plaques were mainly found in cerebral cortex of scrapie-injected mice, the observed changes of SGP-2 mRNA level were not exactly correlated with the site of amyloid piaque
formation. It
has previously shown that SGP-2 mRNA levels were also increased in several nonneural tissues under going programmed cell death and in some tissues of neuronal degeneration. We speculate that the overall increase of SGP-2 mRNA levels in brains of
scrapie-infected mice may be a compensatory response to a neurodegenerative cascade control.
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